![]() The major difference is that an ELISPOT is a combination of both an immunoassay and bioassay because living cells are cultured directly in the wells of the ELISPOT plate.įirst of all, coating antibodies, specific for the protein of interest, are immobilized onto a solid phase (96-well plate). The ELISPOT assay is comparable to an enzyme-linked immunosorbent assay ( ELISA) and is based on the same immunochemical 'sandwich' principle. In general, each cell can be detected by ELISPOT if a characteristic protein is released and specific high affinity antibodies recognizing the protein are available. The assay is a highly sensitive method for the ex vivo quantification of cytokine or antibody secreting cells after stimulation with an appropriate stimulus in vitro ( Cox et al. The enzyme-linked immunospot (ELISPOT) assay is a widely used method for monitoring immune responses. My question is not mentioned here, how can I contact U-CyTech?ĮLISPOT and FluoroSpot assay - general questions Which type of device do I need to evaluate an ELISPOT or FluoroSpot plate? How soon will the spots of the FluoroSpot assay fade? #Dead by daylight gamma control how toHow to dry the plate after color development? Is it possible to stack ELISPOT/FluoroSpot plates in the incubator? What kind of pipette tips should I use for pipetting cells? What are the small, dark spots that are not generated by secreted cytokines or antibodies? Is there an explanation for the stripy appearance of some spots in the T cell ELISPOT assay? What type of spot detection systems are available? Once the ELISPOT plate has been coated, how can it be stored and for how long? How critical are the incubation times mentioned in the ELISPOT/FluoroSpot manual? What are suitable antibodies for the ELISPOT assay? Is there a method to decontaminate the plate when using infectious agents? What about ELISPOT and FluoroSpot assay controls? ![]() How many cells should be added to each well? What should I know about peripheral blood cell preparations? Which cell types can be analyzed by ELISPOT or FluoroSpot assay?Ĭan I use all strains of inbred rodents and non-human primates in the T cell ELISPOT or FluoroSpot assay?Īre there special requirements and storage conditions for blood? What type of information provides an ELISPOT/FluoroSpot assay? ELISPOT and FluoroSpot assay - specific questions In which area of research should an ELISPOT/FluoroSpot assay be used? How does an ELISPOT/FluoroSpot assay differ from an ELISA? Why choose an ELISPOT or FluoroSpot assay? ELISPOT and FluoroSpot assay - general questions For your convenience, we have collected the frequently asked questions (FAQs) on our B cell ELISPOT, T cell ELISPOT assay and FluoroSpot assay. ![]()
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